With the power to rewrite the genetic code underlying countless diseases, CRISPR holds immense promise to revolutionize medicine. But until scientists can deliver its gene-editing machinery safely and ...

Guide Library Design: Researchers design a pooled CRISPR guide RNA library targeting genes of interest. Cell Transduction: The guide library is introduced into cells, typically via lentiviral ...

Artificial intelligence can predict on- and off-target activity of CRISPR tools that target RNA instead of DNA, according to new research published in Nature Biotechnology.

The team suggests TIGER tech could pave the way to the development of precise gene controls for use in CRISPR-based therapies.

A new review highlights the potential of CRISPR–Cas13 for RNA-editing therapies. Discussing the advantages of the effector protein Cas13 over Cas9, they report that CRISPR–Cas13 ...

AI and CRISPR precisely control gene expression RNA-based predictive models developed Date: July 3, 2023 Source: Columbia University School of Engineering and Applied Science Summary: The study ...

Using CRISPR-Cas13 to edit RNA and avoid off-target activity, the researchers systematically profiled nearly 6,200 gene pairs of long noncoding RNAs (lncRNAs) and nearby protein-coding genes ...

MEGA CRISPR: Engineering Better Immunotherapies with RNA Editing Multi-targeted Cas13 screens help researchers avoid permanent DNA cuts and evade T cell exhaustion.

Ancestry Bias Could Cause CRISPR Screens to Veer Off-target Current CRISPR guide RNAs are designed based on European reference genomes, leading to false-negative results in cells from people with ...